Human IFNGR1 ELISA Kit (Serum, Plasma, Cell Lysate, TCM)

Citations
Catalog Number: 41580
  • Accurate quantitation of soluble and membrane Interferon Gamma Receptor 1
  • Compatible with serum, plasma, cell lysates and tissue culture media (TCM)
  • Provides high sensitivity detection of Human IFNGR1 to < 10 pg/ml in Sample Diluent and Cell Lysates
  • < 4 hour assay time
  • Conserve high value specimens with low sample requirements

 

Product Name: VeriKine Human Interferon-Gamma Receptor 1 ELISA Kit

$795.00
Pack Size
Typical Soluble IFN-Gamma Receptor 1 Standard Curves in different matrices using PBL's Human IFNGR1 ELISA (41580)
Typical Soluble IFN-Gamma Receptor 1 Standard Curves in different matrices using PBL's Human IFNGR1 ELISA (41580)

Product Info

Compatibility Serum, Plasma, Cell Lysate, Tissue Culture Media
Assay Range 9.38 - 300 pg/ml (Sample Diluent and Cell Lysates), 234.38 - 7500 pg/ml (Serum and Plasma), 37.5 - 1200 pg/ml (TCM)
Assay Length 3 hours, 15 minutes
Specificity Human IFN-Gamma Receptor 1 (IFNGR1)
CV

Inter-Assay: ≤ 12%


Intra-Assay: ≤ 8%


Spike Recovery: 93% 

 

The VeriKine Human IFN-Gamma Receptor 1 ELISA quantitates Human IFN-Gamma Receptor 1 (IFNGR1)  in serum, plasma, tissue culture media (TCM) and cell lysates by sandwich enzyme linked immunosorbent assay (ELISA). IFNGR1 binds to plates coated with antibody and detection is accomplished using a detection antibody followed by streptavidin conjugated to horseradish peroxidase (HRP). This ELISA kit utilizes Tetramethyl-benzidine (TMB) as the substrate. The standard provided is recombinant Human IFNGR1 expressed in mammalian cells.

 

Documentation

Documentation

Cross-reactivity

No measurable IFNGR1 was observed when using this kit with sera of cynomolgus, rhesus, pig, bovine, rabbit, mouse, sheep or donkey.

 

No cross-reactivity against

  • Mouse IFNGR1-Fc
Storage 2-8°C
Expiration Date One year from date of manufacture
Storage Wet Ice

 

 

Materials Provided:

  • Pre-coated microtiter plate
  • Plate Sealers
  • Wash Solution Concentrate
  • Human IFN-Gamma Receptor 1 (IFNGR1) Standard, 500,000 pg/ml
  • Sample Diluent
  • Antibody Concentrate
  • Antibody Diluent 
  • HRP Conjugate Concentrate 
  • HRP Diluent
  • TMB Substrate 
  • Stop Solution 
 
 

References: (For additional references, please reference the protocol.)

 

  1. Bach EA, Aguet M, and Schreiber RD. "The IFN-g receptor: a paradigm for cytokine receptor signaling." Annu. Rev. Immunol, 1997, 15: 563.

  2. Hawkyard SJ, et al. "The expression of interferon-g receptors on bladder cancer cells: does it correlate with biological response?" Urological Research, 1992, 20: 229-232.

  3. Merlin G, Falcoff E, and Aguet M. I-labelled Human Interferon Alpha, Beta and Gamma: Comparative Receptor-binding Data." J. Gen. Virol., 1985, 66: 1149-1152.

    4. J. Clin. Invest., 1985, 76:2196.

     
  4. Bello I, et al. High levels of soluble IFN gamma receptor alpha chain in the plasma of rheumatoid arthritis patients. Biotherapy, 1998, 11(1): 53-57.

  5. Toren A, et al. "Soluble interferon-gamma receptor and interferon-gamma in patients undergoing allogeneic bone marrow transplantation for hematological malignancies." Cytokines Cell Mol Ther., 1997, 3(3): 153-158.
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