Human Interleukin 15 / IL-15Rα Heterodimer (Human Cell-Expressed)

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Catalog Number: 11702

Recombinant Human IL-15 protein suitable for Direct Binding ELISA, Western Blot (WB), and CTLL-2 Proliferation Assay

$219.00
Pack Size
Reducing SDS-PAGE (Left) and Native PAGE (Right) of PBL's human cell-expressed Human IL-15 / sIL-15Rα Heterodimer (11702)

Reducing SDS-PAGE (Left) and Native PAGE (Right) of PBL's human cell-expressed Human IL-15 / sIL-15Rα Heterodimer (11702)

Reducing Western comparison of PBL's Human IL15/sIL15Ra Heterodimer (PBL) & human IL15 protein from supplier A (Comp A). Probes: anti-human IL15Rα or anti-human IL15 primary Abs

Reducing Western comparison of PBL's Human IL15/sIL15Ra Heterodimer (PBL) & human IL15 protein from supplier A (Comp A). Probes: anti-human IL15Rα or anti-human IL15 primary Abs

Product Info

Tested Applications

  • Direct Binding ELISA
  • Western Blot
  • CTLL-2 Proliferation Assay

Bioactivity

Typically <0.5 ng/ml (15.6 pM), measured by its dose-dependent stimulation of the proliferation of CTLL-2 cells

 

 

Authentic human cell-expression with no tag.

Documentation

Certificate of Analysis (CoA) and Safety Data Sheet (SDS)

Formulation

Phosphate buffered saline (PBS) containing 0.1% bovine serum albumin (BSA)

Molecular Weight

31 kDa predicted (12.8 kDa for IL-15 + 18.4 kDa for sIL-15Rα). Heterodimer exhibits multiple bands on reducing SDS-PAGE due to varying degrees of glycosylation; migrates to a single band at ~87 kDa, observed in Native-PAGE

Source

Recombinant DNA expressed in Human Embryonic Kidney Cells (HEK293)

Purity

Greater than 95% as determined by SDS-PAGE by a combination of ion exchange and size exclusion chromatography
Endotoxin < 1 EU/µg

Storage

For retention of full activity store at -70°C or below and avoid repeated freeze/thaw cycles

Synonyms

IL-15, IL15, sIL-15Rα

Accession Number

NP_002180 (IL-15); NP_002180.1 (sIL-15Rα)

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