Anti-Human IFN Beta Antibody, Clone MMHB-3 (MAb)
Specifications
| Formulation | Supplied frozen in phosphate-buffered saline (PBS) containing 0.1% Bovine Serum Albumin (BSA) |
| Antigen | Human interferon beta |
| Isotype | IgG1 kappa |
| Host Species | Mouse |
| Purity | > 95% by SDS-PAGE stained by Coomassie Blue Endotoxin level < 1 EU/μg |
| Bioactivity | Measured by neutralization of interferon in cytopathic effect inhibition assay. |
| Specificity | Binds to human IFN-beta with high affinity |
| Storage | For retention of full activity store at -20°C or below and avoid repeated freeze/thaw cycles |
| Antigen Synonyms | Human Beta Interferon, Human IFNB, Human Fibroblast IFN, Human IFN Beta, Human Fibroblast Interferon, Human Beta IFN, Human Type I Interferon Beta, Human IFN B |
| Clone | MMHB-3 |
Tech Info / Data
Tested Application:
- Direct Binding ELISA
Suggested Applications*:
- Western Blot
- Flow Cytometry
- Immunoprecipitation
- Immunohistochemistry
*Please note that these applications are presented for suggested use only and have not been fully evaluated by PBL
Documentation
Representative Binding Curve
Representative binding curve of antibody to mammalian expressed human interferon beta 1a (circle) and to 1% BSA/PBS (square) in a Direct Binding ELISA. High-binding polystyrene plates were coated with either 1 µg/ml human interferon beta 1a or 1% BSA/PBS. Thereafter, titrations of the product were added to the wells. Donkey and anti-mouse IgG conjugated to HRP was used as the detection antibody. Colorimetric detection was performed using 3,3',5,5'-Tetramethylbenzidine (TMB) substrate. The HRP-TMB reaction was stopped using a diluted H2SO4/HCl solution.
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