Anti-Human IFN Beta Antibody, Clone MMHB-3 (MAb)

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Product Features: 
  • Mouse monoclonal antibody against Human IFN Beta (Clone MMHB-3)
  • Suitable for use in Direct Binding ELISA 
  • Suggested applications include Western Blot, flow cytometry, immunoprecipitation and immunohistochemistry
  • Unconjugated

Ordering

Catalog No. Pack Size Price Quantity
21400-1

50 μg

 $215.00

Specifications

Formulation Supplied frozen in phosphate-buffered saline (PBS) containing 0.1% Bovine Serum Albumin (BSA)
Antigen Human interferon beta
Isotype IgG1 kappa
Host Species Mouse
Purity > 95% by SDS-PAGE stained by Coomassie Blue
Endotoxin level < 1 EU/μg
Bioactivity Measured by neutralization of interferon in cytopathic effect inhibition assay.
Specificity Binds to human IFN-beta with high affinity
Storage For retention of full activity store at -20°C or below and avoid repeated freeze/thaw cycles
Antigen Synonyms Human Beta Interferon, Human IFNB, Human Fibroblast IFN, Human IFN Beta, Human Fibroblast Interferon, Human Beta IFN, Human Type I Interferon Beta, Human IFN B
Clone MMHB-3

Tech Info / Data

Tested Application:

  • Direct Binding ELISA

Suggested Applications*:

  • Western Blot
  • Flow Cytometry
  • Immunoprecipitation
  • Immunohistochemistry

*Please note that these applications are presented for suggested use only and have not been fully evaluated by PBL

Citations

6 Citations:

  1. Teles, Rosane, et al. (2015). IL-27 suppresses antimicrobial activity in human leprosy. JID, 15 pgs. PMID: 26030183. (link)

  2. Woo, et al. (2013). Developmental Differences in IFN Signaling Affect GATA1s-Induced Megakaryocyte Hyperproliferation. JCI, 14 pgs. PMID: 23863621. (link)

  3. Tamassia, Nicola, et al. (2008). Activation of an immunoregulatory and antiviral gene expression program in poly(I:C)-transfected human neutrophils. Journal of Immunology, 12 pgs. PMID: 18941247. (link)

  4. Moll, Herwig, et al. (2008). Neutralizing Type I Interferon Antibodies trigger an Interferon-Like Response in Endothelial Cells1. Journal of Immunology, 16 pgs. PMID: 18390705. (link)

  5. Reimer, Thornik, et al. (2008). poly(I:C) and LPS induce distinct IRF3 and NF-kappaB signaling during type-I IFN and TNF responses in human macrophages. JLB. PMID: 18252870. (link)

  6. Reimer, Thornik, et al. (2007). Type I IFN induction in response to Listeria monocytogenes in human macrophages: evidence for a differential activation of IFN regulatory factor 3 (IRF3). Journal of Immunology, 13 pgs. PMID: 17617610. (link)

Documentation

Representative Binding Curve

21400-1 Binding Curve

Representative binding curve of antibody to mammalian expressed human interferon beta 1a (circle) and to 1% BSA/PBS (square) in a Direct Binding ELISA. High-binding polystyrene plates were coated with either 1 µg/ml human interferon beta 1a or 1% BSA/PBS. Thereafter, titrations of the product were added to the wells. Donkey and anti-mouse IgG conjugated to HRP was used as the detection antibody. Colorimetric detection was performed using 3,3',5,5'-Tetramethylbenzidine (TMB) substrate. The HRP-TMB reaction was stopped using a diluted H2SO4/HCl solution.

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