Anti-Human IFN Beta Antibody, Clone MMHB-15 (MAb)
Specifications
| Formulation | Mouse monoclonal antibody against human interferon beta, neutralizing |
| Antigen | Human interferon beta |
| Isotype | IgG2b kappa |
| Host Species | Mouse |
| Purity | > 95% by SDS-PAGE stained by Coomassie Blue Endotoxin level < 1 EU/μg |
| Bioactivity | ND50 on human (A549/EMCV) cells is ~27-66 μg/ml in the presence of 96 pg/ml of recombinant human interferon beta 1a, mammalian |
| Specificity | Binds to human IFN-beta with high affinity |
| Storage | For retention of full activity store at -20°C or below and avoid repeated freeze/thaw cycles |
| Antigen Synonyms | None |
| Clone | MMHB-15 |
Tech Info / Data
Tested Application:
- Direct Binding ELISA
Documentation
Representative Binding Curve
Representative binding curve of antibody to mammalian expressed human interferon beta 1a (circle) and to 0.1% BSA/PBS (square) in a Direct Binding ELISA. Medium-binding polystyrene plates were coated with either 1 µg/ml human interferon beta 1a or 0.1% BSA/PBS. Thereafter, titrations of the product were added to the wells. Donkey anti-mouse IgG conjugated to HRP was used as the detection antibody. Colorimetric detection was performed using 3,3',5,5'-Tetramethylbenzidine (TMB) substrate. The HRP-TMB reaction was stopped using a diluted H2SO4/HCl solution.
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