Anti-Human IFN Beta Antibody, Clone MMHB-2 (MAb)

Product Features: 
  • Mouse monoclonal antibody against Human IFN Beta (Clone MMHB-2)
  • Suitable for use in neutralization, direct ELISA and Western Blot
  • Unconjugated

Ordering

Catalog No. Pack Size Price Quantity
21410-1

500 μg

$1,715.00

Specifications

Formulation Supplied lyophilized in phosphate-buffered saline (PBS) containing 5% trehalose prior to lyophilization
Antigen Human interferon beta
Isotype IgG1
Host Species Mouse
Purity Determined by protein A or G affinity chromatography
Bioactivity Measured by neutraliziation of interferon in antiviral assay; ND50 on human (HeLa/EMCV) cells is ~7-21 μg/ml in the presence of 10 ng/ml of human IFN-beta.
Specificity Neutralizes human IFN-beta; does not neutralize human IFN-alpha or gamma
Storage For retention of full activity store at -20°C or below and avoid repeated freeze/thaw cycles
Antigen Synonyms Human Beta Interferon, Human IFNB, Human Fibroblast IFN, Human IFN Beta, Human Fibroblast Interferon, Human Beta IFN, Human Type I Interferon Beta, Human IFN B
Clone MMHB-2

Tech Info / Data

21410-1 Cytopathic Effect Reduction

Recommended Applications:

  • Neutralization
  • Direct ELISA: 0.5 - 1.0 μg/ml
  • Western Blot: 1 - 2 μg/ml

Citations

Neutralization of Hu-IFN-β

21410-1 Neutralization of Hu-IFN-β

To measure the ability of the antibody to neutralize the bioactivity of the human interferon beta on HeLa cells, Hu-IFN-ß was added to various concentrations of the antibody. The antigen-antibody mixture was added to confluent cultures of the HeLa cells in a 96-well plate. The assay mixture in a total volume of 100 µl, containing antibody at the concentrations as indicated, Hu-IFN-ß at 10 ng/ml, was incubated at 37ºC for 20-24 hours in a humidified CO2 incubator. At the end of this incubation period, medium was aspirated from all wells and an appropriate titrated amount of the EMCV in pre-warmed culture medium was added to each test well. After another 20-24 hour incubation, the cells were fixed, stained and scored for cytopathic effect by measurement of optical densities in a microplate reader at 540 nm. The ND50 of the antibody is approximately 7-21 µg/ml.

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