Materials Provided

• Pre-coated microtiter plate
• Plate Sealers
• Wash Solution Concentrate
• Mouse Interferon Beta Standard, 500,000 pg/ml
• Dilution Buffer 
• Antibody Concentrate 
• HRP Conjugate Concentrate
• Concentrate Diluent
• TMB Substrate 
• Stop Solution 

Additional Materials Required (Not Provided) 

• Microplate reader capable of reading an OD at a wavelength of 450 nm
• Variable volume microtiter pipettes
• Adjustable multichannel pipette (50-300 μl)
• Reagent reservoirs
• Wash bottle or plate washing system
• Distilled or deionized water
• Serological pipettes (1, 5, 10 or 25 ml)
• Disposable pipette tips (polypropylene)
• Plate shaker

Tips, Tools and Troubleshooting:

• Importance of ELISA Standard Curve

Background

Interferons (IFNs) are a group of cytokines which exhibit pleitropic activities that play major roles in both innate and adaptive immunity. Type 1 IFNs consist of multiple Interferon Alpha (IFN-α) genes and at least one Interferon Beta (IFN-β) gene in most vertebrates.

IFN-β is primarily known for its antiviral activity, but also has anti-proliferative, anti-inflammatory and immune-modulatory effects. It plays a pivotal role in the protective response to many infections and diseases, but when over expressed or produced unchecked it can also contribute to the generation of clinically relevant side effects and pathological processes. IFN-β is a common therapeutic treatment for multiple sclerosis and some cancers with the research into these diseases often conducted in mice.

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Background Literature:

1. Staehelin et al. (1981) Methods in Enzymology, Vol. 79. (Pestka, ed.), Academic Press, New York, 589-595.
2. Balachandran et al. (2004) Nature, Vol. 432, 401-405.