Powered by BiozMouse Interferon Alpha 1 protein with antiviral, antiproliferative, and immunomodulatory activities.
Comparison of Mouse IFN-Alpha 1 and Mouse IFN-Alpha A Antiviral Activity. The activity of Mouse IFN Alpha 1 and Mouse IFN Alpha A was compared in an L929/EMCV CPE assay. The EC50 for Mouse IFN Alpha 1 in this experiment was 62 pg/ml.
Product Info
Mouse IFN-Alpha 1 is an interferon with broad tissue expression which may be highly effective against certain viruses such as CMV and HSV.
Documentation
Certificate of Analysis (CoA) and Safety Data Sheet (SDS)
12105-1 CoA
12105-1 Certificate of Analysis (CoA)
12105 SDS
12105 Safety Data Sheet
| Formulation | Supplied frozen in phosphate-buffered saline (PBS) containing 0.1% Bovine Serum Albumin (BSA) |
|---|---|
| Molecular Weight | 19.1 kDa |
| Source | Gene obtained from mouse DNA expressed in E.coli |
| Purity | > 95% by SDS-PAGE stained by Coomassie Blue Endotoxin level < 1 EU/μg |
| Bioactivity | Measured using a cytopathic inhibition assay on Mouse (L929) cells with EMCV. The EC50 for IFN in this assay is ~ 5 U/ml. |
| Storage | For retention of full activity store at -70oC or below and avoid repeated freeze/thaw cycles |
| Synonyms | Mu IFN-α1, Mouse IFN Alpha Subtype, Mouse IFN Protein Subtype, Interferon Alpha, IFN Alpha, IFN-a, Type I IFN Alpha |
| Accession Number | AY225950 |
Background
Mouse IFN-Alpha 1 was originally cloned from a genomic library by Zwarthoff et al. [(1985) Nuc. Acids Res. 13(3)791]. It is produced by macrophages, neutrophils, plasmacytoid dendritic cells (pDCs) and other somatic cells in response to many viral and some pathogen infections.
Expression of the Mouse IFN-Alpha 1 subtype has been seen in embryonic fibroblasts in some studies, but not in others. Expression has also been observed in brain and in L929 cells challenged with a variety of viruses [Yeow et al. (1997) Int. J. Biochem. Cell Biol. 29(3):519, Barchet et al., (2002) J. Exp. Med. 195(4):507, Delhaye et al., (2006) PNAS. 103(20):7835, Fung et al., (2004) J. Imm. Meth. 284:177]. It has been suggested that expression of this IFN may be regulated by Oct-1 and that it is IRF-7 dependent [Mesplede et al., (2005) Mol. Cell. 25(19):8717, Sato et al., (2000) Immunity 13:539].
This subtype has been extensively studied either in transgenic mice or by delivery as an expression construct [Hekman et al., (1988) J. Biol. Chem. 263(24):12151, Carr et al., (1988) J. Immunol. 169(9):5859]. When delivered as an expression construct, Mouse IFN-Alpha 1 reduced CMV intramuscular infection significantly better than Mouse IFN-Alpha 4 or 9 [Yeow et al., (1988) J. Immunol. 160(6):2932].
Summary: Mouse IFN-Alpha 1 is an interferon with broad tissue expression which may be highly effective against certain viruses such as CMV and HSV.
For more information on Mouse IFN-Alpha subtype nomenclature click here.