Mouse IFN-Alpha 13
Catalog Number: 12130
Recombinant Mouse Interferon Alpha 13 protein expressed in E.coli. Member of the Mouse IFN-Alpha family which may be constitutively expressed and thus have different expression criteria than other subtypes.
$365.00
Product Info
Mouse IFN-Alpha 13 is a new interferon which may be constitutively expressed and thus have different expression criteria than other Mu IFN-Alpha subtypes.
Specifications
| Formulation | Supplied frozen in phosphate-buffered saline (PBS) containing 0.1% Bovine Serum Albumin (BSA) |
|---|---|
| Molecular Weight | 19.1 kDa |
| Source | cDNA obtained from mouse leukocyte interferon expressed in E.coli |
| Purity | > 95% by SDS-PAGE stained by Coomassie Blue Endotoxin level < 1 EU/μg |
| Bioactivity | Measured using a cytopathic inhibition assay on Mouse (L929) cells with EMCV. The EC50 for IFN in this assay is ~ 5 U/ml. |
| Storage | For retention of full activity store at -70°C or below and avoid repeated freeze/thaw cycles |
| Synonyms | Mu IFN-α13, Mouse IFN Alpha Subtype, Mouse IFN Protein Subtype, Interferon Alpha, IFN Alpha, IFN-a, Type I IFN Alpha |
| Accession Number | AY220461 |
Tech Info & Data
Background
Mouse IFN-Alpha 13 was recently cloned from cells infected with Theiler's virus [van Pesch and Michiels (2003) J. Biol. Chem., 278(47):46321] and from genomic DNA [Hardy et al. (2004) Genomics 84(2):331]. Van Pesch and Michiels had suggested that Mouse IFN Alpha 13 is expressed constitutively in L929 cells. They have also shown that priming of the cells with IFN alpha/beta leads to a decrease in this transcript.
Summary: Mouse IFN-Alpha 13 is a new interferon which may be constitutively expressed and thus have different expression criteria than other Mu IFN-Alpha subtypes.
For more information on Mouse IFN-Alpha subtype nomenclature click here.
Citations
References
- van Pesch and Michiels (2003) J. Biol. Chem. 278 (47):46321.
- Hardy et al. (2004) Genomics 84(2):331.