This Human IFN-Alpha ELISA kit detects 11 of 12 major IFN-Alpha subtypes in serum, plasma, and tissue culture media (TCM).
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Product Name: VeriKine Human Interferon-Alpha Multi-Subtype ELISA Kit (Serum, Plasma,TCM)
Representative IFN-Alpha Standard Curve from 12.5 pg/ml to 1000 pg/ml in Standard Diluent Using PBL's Human IFN-Alpha Multi-Subtype Serum ELISA (41110)
Spike Recovery of IFN-Alpha in Different Matrices using PBL's Human IFN-Alpha Multi-Subtype Serum ELISA (41110)
| Matrix Compatibility | Serum, Plasma, Tissue Culture Media |
|---|---|
| Assay Range | 12.5 - 1000 pg/ml |
| Assay Length | 3 hours, 15 minutes |
| Specificity | Human Interferon Alpha |
The VeriKine Human IFN-Alpha Multi-Subtype Serum ELISA kit is designed to provide accurate measurement of human IFN-Alpha amounts in serum and plasma samples. This kit quantitates human IFN-Alpha in human sera, plasma and tissue culture media using a sandwich immunoassay. The kit is based on an ELISA with anti-detection antibody conjugated to horseradish peroxidase (HRP). Tetramethyl-benzidine (TMB) is the substrate. The assay is based on the international reference standard for human interferon alpha (Hu-IFN-a) provided by the National Institutes of Health.
*For global subtype detection and additional sensitivity, we recommend our Human IFN-Alpha All-Subtype TCM ELISA Kit, High Sensitivity (Cat. No. 41135-1).
*For global subtype detection, additional sensitivity, and complex biological matrices such as plasma and serum, we recommend our Human IFN-Alpha All-Subtype ELISA Kit, High Sensitivity (Cat. No. 41115-1).
Protocol, Certificate of Analysis (CoA), Safety Data Sheet (SDS)
41110-1 Certificate of Analysis (CoA) & Protocol (One-Page)
41110-2 Certificate of Analysis (CoA) & Protocol (One-Page)
41110 Safety Data Sheet
COA Sample Diluent Buffer Correction
| CVs and Spike Recovery | Inter-Assay: ≤ 8%
|
|---|---|
| Cross-reactivity | No cross-reactivity against
|
| Synonyms | IFN alpha, IFN-a, interferon alpha, IFN alpha 2, IFN-a2, interferon alpha 2, Leukocyte interferon, Leukocyte IFN, alpha interferon, interferon alfa, IFN alfa, interferon alpha 2a, IFN alpha 2a, interferon a, interferon alpha 2b, IFN alpha 2b, Type I interferon alpha, Type I IFN alpha |
| Storage | 2-8°C |
| Expiration Date | Twenty-one months from the date of manufacture |
| Shipping Condition | Wet Ice |
Spike Recovery of IFN-Alpha in Different Matrices | |||
|---|---|---|---|
| Low (30 pg/ml) | Medium (300 pg/ml) | High (800 pg/ml) | |
| Normal Human Serum | 94% | 100% | 96% |
Normal Human Plasma (Na-Citrate) | 70% | 112% | 110% |
Normal Human Plasma (Na-Heparin) | 95% | 106% | 101% |
Normal Human Plasma (Na-EDTA) | 60% | 86% | 84% |
Interferons (IFNs) are a family of cytokines produced by macrophages, neutrophils, dendritic cells and other somatic cells in response to viruses and pathogens. Interferon Alpha (IFN-alpha), a type I interferon, was one of the first FDA-approved biotherapeutic treatments. Additionally, there is a growing amount of evidence suggesting IFN-alpha may be involved in certain autoimmune diseases such as Systemic Lupus Erythematosus (SLE). Accurate and reproducible detection of IFN-alpha in human serum or plasma plays an important role in advancing immunological investigation and drug discovery.
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