PBL Assay Science offers two lines of human IFN-Beta ELISA kits: VeriKine-HS (41415, 41435), high-sensitivity assays that quantify total IFN-Beta down to 1.2 pg/ml in healthy-donor, autoimmune, and pharmacokinetic serum samples, and VeriKine (41410), a standard-sensitivity assay (LLOQ 50 pg/ml) for tissue culture media where IFN-Beta is readily detectable. Choose VeriKine-HS when near-basal or low-level IFN-Beta must be resolved, and VeriKine when confirming and quantifying a strong induction is the goal.
VeriKine-HS (41435, 41415) High Sensitivity
VeriKine-HS kits detect IFN-Beta down to 1.2 pg/mL (41415) or 2.34 pg/mL (41435), roughly 20 to 40 times more sensitive than the standard kit, for situations where basal or low-level IFN-Beta needs to be resolved rather than just confirmed. This matters most for healthy-donor baseline work, pharmacokinetic monitoring, and autoimmune serum studies, where circulating IFN-Beta typically sits well below what a typical ELISA kit can detect. Both kits are validated to show no inhibition of IFN-Beta detection in the presence of a 3-log excess of soluble IFN-Alpha/Beta Receptor 2 (sIFNAR2), meaning they measure total IFN-Beta rather than being masked by the naturally occurring soluble receptor.
41415 has been validated in real-world disease serum, not just spiked or buffer-based samples. It's been directly validated for quantifying IFN-Beta in autoimmune sera, including from patients with conditions like multiple sclerosis, and for measuring trademarked IFN-Beta 1a and 1b therapeutic molecules in human serum. This makes it suitable for both biomarker studies and pharmacokinetic monitoring of IFN-Beta drug products in actual patient samples (see Technical Literature below).
VeriKine (41410) Standard Sensitivity
The VeriKine kit is a highly cited, well-established assay built for tissue culture media samples where IFN-Beta is present at typical, readily detectable levels, for example following viral infection or interferon-inducing stimulation of cultured cells. With an LLOQ of 50 pg/ml and an assay range of 50–4,000 pg/ml, it's a straightforward fit for induction and screening work where resolving trace-level IFN-Beta isn't the goal.